Field experiments were conducted for two successive seasons of 2007/2008  and 2008/2009 at the Experimental Research Station of the Desert Research Center at El-Kharga Oasis, the New Valley governorate. The object of this work was to study the effect of bio, nitrogen and phosphorus fertilizers on the growth, yield and yield components on canola crop. The results showed that the application of biofertilizers (Azotobacter and Bacillus megatherium caused significant increases in seed oil % in seeds and total oil yield (kg/fed) in the first season while plant height 1000-seed weight and seed yield were not significantly affected by biofertilizers application. In the second season, plant growth yield and yield components were not significantly increased. Nitrogen fertilization showed significant increases on all parameters studied in the two seasons. Data also showed that increasing nitrogen level from 30 to 90 kg N/fed led to significant increases in plant height by 15.9 and 16.3 % in the first and the second seasons respectively. Increasing phosphorus level from 0 to 45 kg P₂O₅/fed resulted in significant increases in seed yield by 15.5 % and 16.3 % in the first and the second seasons respectively.