Stevioside is natural sweetener isolated from the leaves of plant stevia and it is up to 300 times sweetener than sucrose, since it is a sweetener with no caloric value and with proven non-toxic effect on human health. Steviol glycosides are used as a sweetener in many industrial foods, such as soft drinks or fruit juices. So, two field experiments were conducted at Mallawi Agricultural Research Station, El-Minia Governorate during 2014 and 2015 seasons to deduce the effect of different sources of nutrient, i.e. Control (80 kg N fed^-1), K-humate + (40 kg N fed^-1), biofertilizer +(40 kg N fed^-1) and compost + (40 kg N fed^-1)] and number of cutting , i.e. 1^st , 2^nd  and 3^rd cutting on yield and quality of stevia (Stevia rebaudianaBertoni) under Middle Egypt conditions. The obtained data pointed out that different sources of nutrient had a significant effect on plant height, fresh plant weight, dry plant weight, fresh leaves weight/plant, dry leaves weight/plant, N, P and K % of stevia leaf, fresh biomass yield, dry biomass yield , fresh leaves yield, dry leaves yield and fresh stem yield, total stevioside % (St %), rebaudioside A% (Rb%), stevioside yield and rebaudioside A yield of stevia in two growing seasons.
The studied cutting number of stevia had a significant influence on plant height, fresh plant weight, dry plant weight, fresh leaves weight/plant, dry leaves weight/plant, dry leaves weight: dry stem weight, P % of stevia leaf, fresh biomass yield, dry biomass yield, fresh leaves yield, dry leaves yield  and fresh stem yield and dry stem yield of stevia, rebaudioside A%, total stevioside yield (kg/fed) and rebaudioside A yield (kg/fed) of stevia rebaudiana in two growing seasons. From the present study, it may be concluded that the application of compost at 2.0 ton/fed+(40 kg N fed-1) with 3^rd cutting was the best treatment for improving the yield and quality of stevia (Stevia rebaudianaBertoni) and is advisable because it is achieved the highest value of rebaudioside A(102.80 kg/fed) of stevia. This helps in reducing the great gap in sugar production, which amounted to 0.7 million tons between production and sugar consumption at the national level production under the experimental conditions.