This investigation was carried out during 2015 & 2016 seasons, at Afak farm located at Balat district, New Valley Governorate. To study the effect of yeast and bio-power vegetative growth, nutrient status, and fruiting of Flame Seedless grapevines. The experiment was arranged in a complete randomized block design with seven treatments and three replications consisting of two vines per each. The obtained results could be summarized as follow: Using the recommended dose of nitrogen (RDN) via 25% mineral plus 75% bio-form significantly increased the number of leaves/shoot and leaf area as well as leaf nutrient composition compared to use RDN via mineral N fertilizer alone. No significant differences on these traits due to use RDN via bio-form plus either 75 or 50% mineral-N. Also, no significant differences on shoot length due to use the double or triple form of fertilization compared to use mineral-N only. All combined fertilization treatments significantly increased the yield and improved the cluster and berry traits compared to use RDN via mineral source only. It is evident from the foregoing results that double form (50% plus 50% yeast or bio-power) or triple form of fertilization (25% m + 25 or 50% yeast plus 50 or 25% bio-power) improved the vegetative growth, yield and berry quality. In addition, it minimized the production costs and environmental pollution