Plant diseases caused by insect-transmitted viruses are among the most serious potato production problem. Alfalfa mosaic virus (AMV) and Potato virus Y (PVY) consider among the most common viruses greatly infecting potato crop in Assiut governorate and result in a serious losses in potato production as well as reduction of potato quality. Results revealed that using meristem culture technique alone was not sufficient to produce virus free potato plantlets, but when this technique integrated with thermotherapy it proved high efficiency to eradicate the viral agents.Also, aqueous plant extract of Barnoof (Pluchea discorodis) in the nutrient media used in meristem culture technique (Murashige and Skoog) could be useful to increase the efficiency of this technique to produce  virus free  plantlets. Culture filtrates of Oyster mushroom Pleurotus ostreatus L. or entomopathogenic fungus  Ascosphaera apis  have an inhibitory effects against Alfalfa mosaic virus and  using these culture filtrates either separately or simultaneously resulted in significant reduction of local lesions produced in indicator plants Chenopodium  sp.