Application of nitrogen (N) fertilizer is one of the most important actions that increases grain yield and improves grain quality in winter wheat (Triticum aestivum L.) production. Presently, there are a large number of investigations in the field on different nitrogen fertilizer application regimes. However, there is little information available about the optimum level of N for drip irrigated wheat. Filed experiments in Randomized Complete Block Design (RCBD) conducted to study the response of drip irrigated wheat to three levels of N (120, 180, and 240 kg N ha⁻¹). The field experiments were carried out at the Agricultural Experimental Station farm of the Faculty of Agriculture, Assiut University, Egypt during the two successive growth seasons  of 2013/14 and 2014/2015. The application of N significantly (P<0.05) enhanced the growth of drip irrigated wheat. The highest rate of nitrogen increased the uptake of N, P, and K by 61, 75, and 57% compared to the lowest treatment. Increasing rate of nitrogen to 240 kg N per hectare increased the Water Use Efficiency (WUE) by 34 and 22% in the first and second season, respectively, compared to N₁₂₀ treatment. The number of spike per m², number of grains per spike, and grain weight per spike of wheat were affected significantly (P<0.05) by N treatments. The application of N₂₄₀ increased the wheat grain yield by 34 and 22% in the first and second season, respectively, compared to N₁₂₀. Based on the obtained results, it is recommended to fertilize drip irrigated wheat by 240 kg N per hectare.