A field experiment was carried out at The Experimental Farm, Faculty of Agriculture, Al-Azhar Univ. Assiut, Egypt in order to study the effect of different nitrogen fertilization (bio, organic and chemical) with different combination on sunflower yield and its oil content as well as the damage caused by wild birds. The fertilization treatments were carried out as follow. The first treatment without any fertilization as control. The second was treated with ammonium nitrate as a chemical fertilizer (CN) with rate of 50 Kg/fed the third treatment was the effective microorganisms (EM) with rate 6 L/fed. as a bio-fertilizer.The fourth treatment was treated with humic acid (HA) with a rate of 4 L/fed + EM (EMH). The fifth area was treated with a rate of 20 m³ fed from the sheep manure (SM) as organic fertilizer. The sixth treatment was a mixed from SM + EM. The last treatment was mixture from SM + EMH. Each treatment was three plots every one was (2 × 4.5m.) and laid out in a randomized block design with three replicates