Damping-off disease caused by certain Fusarium spp. and other fungal species is an important diseases of wheat. Isolations from infected wheat seedling revealed that presenceofFusarium oxysporum, Fusarium chlamydosprum, Fusarium lateritium, Fusarium proliferatum, Fusarium equiseti, Rhizoctonia solani and Macrophomina phaseolina. This pathogens were able to infect Giza-168 and Banyswif-1 wheat cultivars causing damping-off. The antagonistic capability of the isolated fungi against the pathogens revealed that 11 fungal isolates out  of 53 tested isolates showed moderately and highly antagonistic effect against all tested pathogenic fungi. Two Trichoderma harzianum isolates gave over growth upon the mycelia growth of the pathogens. Soil treatment with T.harzianum gave significantly reduction in incidence of damping-off on Giza-168 and Banyswif-1 cultivars under greenhouse conditions.