In the present investigation, 12 diverse genotypes of sugarcane (Saccharum officinarum L.) were studied to assess their genetic potential for yield, yield contributing characters and juice quality traits. The mean squares of years, genotypes and their interaction were highly significant in all studied traits, except years and interaction of stalk diameter. The phenotypic and genotypic coefficient of variation (GCV and PCV) was moderate to high for stalk height, stalk weight, number of stalks/m², cane yield, sugar yield, and reducers %. Low values of GCV and PCV were found in stalk diameter, recoverable sucrose %, Brix (%), Pol %, Purity (%) and Fibers %, which indicating lower variability in these traits. Maximum genetic gain along with high heritability was observed for stalk height, cane yield, stalk weight, and sugar yield. Reducers% showed high genetic gain and moderate heritability while, number of stalks/m² showed moderate genetic gain and heritability estimates. High amount of heritability associated with low to moderate genetic gain were observed for Purity% and stalk diameter. Low genetic gain with moderate amount of heritability were observed for recoverable sucrose %, Brix%, Pol%, and Fibre%.