Nile river Delta contains the major agricultural and aquacultural base lands in Egypt. Fish farming lands are spread in the northern part of the Nile Delta. Soil/sediments in fish ponds of the Delta are drained and disposed for improving the conditions of bottoms. Land uses include upland cultivation, and short- and long-term of catfish production. It was worthy to examine the influence of fish land use on some soil properties and the availability of some soil micronutrients. Ten soil profiles were chosen to represent the most popular uses of soils in the northern Nile part of the Delta including fish farming. A virgin soil was used as a reference for making comparison among the different practices. The available content of soil Fe, Mn and Cu and their relation with soil properties were studied. The results showed that the available Mn, Fe, Cu, and Zn in the catfish pond soils were higher than north upland cultivated and virgin soils. Available Fe, Mn, Cu and Zn decreased with increasing the duration use of catfish pond soils and cultivated soils, while available Zn increased with increasing duration of cultivated soil. Available Fe, Mn, Cu and Zn showed significant correlations with soil properties. The results may be applicable to other river delta areas of the world.