593

The Effect of Picloram and Thidiazuron Concentrations on Prolif-eration Somatic Embryos from Immature Inflorescence of Date palm

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Horticulture (Vegetable, Pomology and Ornamentals)

Abstract

Immature inflorescences of date palm(Phoenix dactylifer) cv.Sewi were used as explants for in vitro culture to investigate the effect of plant growth regulators oninflorescence proliferation. The inflorescences on initiation stage cultured on solidified Murashige and Skoog (MS) basal medium supplemented withPicloram 1, 5, 10 and 15 mg l-1 for two re-cultures. Then, transferred into medium supplemented with different concentrations of TDZ combined with NAA on proliferate stage. The optimal concentrations for successful inflorescence growth was 5 or 10 mg l-1 Picloram and through studying the residuals effect of Picloram on inflorescences proliferation in the presence of three concentrations of TDZ, it found that, 0.5 mg l-1 TDZ combined with 0.1 mg l-1 NAA was more effective to induce direct somatic embryos and gave the highest inflorescence proliferation percentage, while the high level of Picloram induced callus. Vegetative shoots formed into media containing 0.1 NAA mg l-1, 0.2 mg l-1 BA and 0.2 mg l-1 kin. All somatic embryos were converted successfully to healthy normal plantlets which could be transferred to greenhouse.

DOI

10.21608/ajas.2014.593

Keywords

Phoenix dactylifera, in vitro inflorescences, direct somatic embryos, Picloram, TDZ

Authors

First Name

Rehab

Last Name

Sidky

MiddleName

A.

Affiliation

The Central laboratory of Date palm Researches and Development, Agric. Research Center, Egypt.

Email

sidky1234rehab@hotmail.com

City

-

Orcid

-

Volume

45

Article Issue

1

Related Issue

155

Issue Date

2014-03-01

Receive Date

2014-01-01

Publish Date

2014-03-01

Page Start

58

Page End

67

Print ISSN

1110-0486

Online ISSN

2356-9840

Link

https://ajas.journals.ekb.eg/article_593.html

Detail API

https://ajas.journals.ekb.eg/service?article_code=593

Order

3

Type

Original Article

Type Code

62

Publication Type

Journal

Publication Title

Assiut Journal of Agricultural Sciences

Publication Link

https://ajas.journals.ekb.eg/

MainTitle

The Effect of Picloram and Thidiazuron Concentrations on Prolif-eration Somatic Embryos from Immature Inflorescence of Date palm

Details

Type

Article

Created At

22 Jan 2023