Beta
558

Genetic Diversity in Wheat Genotypes Using Random Amplified Polymorphic DNA (RAPD)

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Genetics

Abstract

Genetic diversity among 75 wheat genotypes (66 hexaploids and 9 tetraploids) was studied using 6 RAPD primers. A total of 76 DNA bands were generated by the 6 RAPD primers from all genotypes, of which 53 (70.23%) were polymorphic. The total number of amplification products per primer ranged from 6 (OPO06) to 16 (UBC03 and UBC09) with an average of 12.67 bands per primer. The size of PCR products ranged from 1478bp (OPA01) to 91bp (UBC03). A significant correlation (0.892, p<0.01) was observed between the total number of bands and the number of polymorphic bands. The six primers amplified 11 unique bands for specific genotypes that would prove to be highly useful in genotype identification and designing the future breeding strategy. The similarity coefficient values ranged from 0.68 to 1.00, indicating moderate to high genetic similarityin tested loci among the wheat genotypes. The 75 wheat genotypes were grouped into two major clusters within the dendrogram. Cluster I had the tetraploid genotypes and the hexaploid genotypes were placed in cluster II.

DOI

10.21608/ajas.2015.558

Keywords

Wheat, RAPD, Genetic diversity, PCR, varietal identification

Volume

46

Article Issue

2

Related Issue

149

Issue Date

2015-04-01

Receive Date

2015-03-18

Publish Date

2015-04-01

Page Start

50

Page End

70

Print ISSN

1110-0486

Online ISSN

2356-9840

Link

https://ajas.journals.ekb.eg/article_558.html

Detail API

https://ajas.journals.ekb.eg/service?article_code=558

Order

5

Type

Original Article

Type Code

62

Publication Type

Journal

Publication Title

Assiut Journal of Agricultural Sciences

Publication Link

https://ajas.journals.ekb.eg/

MainTitle

Genetic Diversity in Wheat Genotypes Using Random Amplified Polymorphic DNA (RAPD)

Details

Type

Article

Created At

22 Jan 2023