This study aimed to evaluate yield performance of cotton genotypes in different environments to know the best genotypes for produce the new variety. Thirty-eight new cotton lines descending from fifteen Egyptian cotton crosses were included in Trail A, and eighteen lines descending from thirteen crosses were included in Trail B in 2014 season using two commercial varieties Giza 80 and Giza 90 as checks. Trail A was raised at Seds, whereas Trail B was held at five locations (Seds, El-Fayoum, El-Menia, Assuit and Sohag) in Middle and Upper Egypt. The results of Trail A showed that the most of genotypes belonging to crosses exceeded significantly the check variety Giza 80 in both yield and its contributing variables. While, Trial B showed the superior of the nine crosses i.e. No. 1([{(G.83XG.80) x Dendara}x(G91xG90)]), 5(G.80 x (G.91 x G.90)), 6(G.80 x (G.91 x G.90)), 7(G.80 x (G.91 x G.90)), 8(G.80 x (G.91 x G.90)), 9([G.83 Radiator x Aust.) x G.91), 14([{(G.83 x G.80) x G.89}x (G.83 x Deltbain 703)), 15([{(G.83 x G.80) x G.89} x Aust.]) and No. 17([{G.83 x (G.75 x 5844)} x G.80]) surpassed significantly the check variety Giza 90 in seed cotton yield and lint yield, moreover one of them No. 14([{(G.83 x G.80) x G.89}x (G.83 x Deltbain 703)) significantly exceeded Giza 90 in boll weight across five locations. High heritability estimates in broad sense were recorded for most studied traits in Trail A and Trial B indicating that phenotypic selection for these strains could be highly effective. High gcv estimates were observed in seed cotton yield (9.66 and 30.08%), lint yield (10.93 and 33.17%) and number of bolls (3.16 and 2.21%) in Trial A and Trial B, respectively. The significant genotypes x locations interaction indicated that, such materials were affected by the environment and it should further be evaluated for several years at different locations