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274355

Eco-Friendly Microbial Fungicide of Bacillus spp., Trichoderma album, and Saccharomyces cerevisiae on Rhizoctonia solani, the Causal Agent of Potato Black Scurf Disease

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Plant diseases

Abstract

Microbial biocontrol agents against Rhizoctonia solani (Rh), the causal agent of potato black scurf disease investigated. Endophyte Bacillus subtilis (Bac1 and Bac2), the fungal bioagents Trichoderma album, and Saccharomyces cerevisiae (yeast) were tested for their ability to inhibit the growth of R. solani using the dual culture technique. Four isolates of R. solani from infected potato tubers were collected from different areas in Alexandria and Behira governorates. All fungal isolates were identified by microscopy and confirmed with distinct morphologies. Molecular identification, DNA isolation, and ITS amplification were carried out on the tested R. solani isolates. The pathogenicity test of the R. solani isolates approved their ability to inhibit radish seeds germination on the water agar media compared to the control treatment in which the % of seed germination was 100%. Pathogenicity on potato plants also confirmed the isolates' potential. Isolate R. solani (Rh1) outperformed the others in terms of pathogenicity, severity on Cara and Spunta cultivars were 87.5% and 81.25%, respectively. As well, the Rh1 isolate exceeds the other isolates in increasing the colored area of potato leaves of the Cara cultivar.  Co-culturing B. subtilis and R. solani isolate on PDA resulted in high inhibition rates for all isolates. T. album exhibited inhibition of all R. solani isolates tested on PDA at the rate of 2 according to the scale of Bell et. al., 1982. Scanning electron micrographs showed that T. album hyphae aggregated, penetrated, and deformed the hyphae of R. solani.S. cerevisiae was demonstrated to inhibit all isolates of R. solani on PDA medium when compared to the control treatment, which had a rate of inhibition of 0.00%. Thus, the current study demonstrated the efficacy of endophytes B. subtilis, T. album, and S. cerevisiae in limiting R. solani growth in vitro.
 

DOI

10.21608/asejaiqjsae.2022.274355

Keywords

Eco-friendly, Biocontrol, Bioagents, Rhizoctonia solani, Endophytes, Bacillus subtilis, Trichoderma album, and Saccharomyces cerevisiae

Authors

First Name

Ola

Last Name

Jaafar

MiddleName

Hidi

Affiliation

Plant Pathology,Faculty of Agriculture, Alexandria, Egypt

Email

olaahadi18@gmail.com

City

Alexandria

Orcid

-

First Name

Sayed

Last Name

S. Aboshosha

MiddleName

-

Affiliation

Department of Plant Pathology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

Email

sayed.aboshosha@alexu.edu.eg

City

-

Orcid

-

First Name

Alia

Last Name

Shoeib

MiddleName

Abed El-Baky

Affiliation

Plant Pathology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

Email

aliashoeib@alexu.edu.eg

City

Alexandria

Orcid

0000-0002-7075-2780

First Name

Amany

Last Name

H. M. Shams

MiddleName

-

Affiliation

Department of Plant Pathology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

Email

amany.shams@alexu.edu.eg

City

-

Orcid

-

Volume

43

Article Issue

4

Related Issue

37463

Issue Date

2022-12-01

Receive Date

2022-11-10

Publish Date

2022-12-30

Page Start

637

Page End

649

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 43_Issue 4_Pages 637-649.pdf

PDF . 1.0MB

Link

https://asejaiqjsae.journals.ekb.eg/article_274355.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=274355

Order

9

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Eco-Friendly Microbial Fungicide of Bacillus spp., Trichoderma album, and Saccharomyces cerevisiae on Rhizoctonia solani, the Causal Agent of Potato Black Scurf Disease

Details

Type

Article

Created At

22 Jan 2023