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240155

Regeneration, in Vitro Mutation and Evaluation of Genetic Stability of Gardenia Somaclones Via Ssr Markers

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Last updated: 01 Jan 2025

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Tags

Horticulture

Abstract

The aim of this research was to study the possibility of creating genetic differences in Gardenia plant to obtain a new variety with high economic value. To achieve this goal, three laboratory experiments were conducted as follows:
1-     In vitro propagation: The results indicated that the axillary buds when cultured on medium consisting of MS + 0, 5 of BA+ 0.1 mg l-1 of Kin. gave the highest significant results for leaves number 25.4, shoot number 3.28 and shoot length 3.1 cm. The highest significant values of root number and length 6.65 and 1.3 cm, respectively ​​were recorded when shoots were transferred to MS medium combined with 0.75 mg l-1 of IBA.
2-     In vitro mutation: Results indicated that the degree of variegated leaves increased by increasing the concentration of the mutagenEthylmethanesulphonate (EMS) up to 0.4 %, but treatment with a concentration of 0.5% led to explants death or deformation of the leaves.
3-     Molecular markers Experiment: An experiment was conducted to examine the applicability of SSR markers to discover the polymorphisms of mutations caused by EMS and their genetic relationships for the first time. Thirteen SSR primers were used. Plants were divided into five groups, namely the original plant and the mutant plants resulting from growth on the five EMS concentrations (0.0, 0.1, 0.2, 0.3 and 0.4%). Cluster analysis was done for all the genotypes under study. Results indicated that 24 bands were obtained, their size ranged between 50: 250 pb, and all of them were 100% Polymorphic. PIC values ​​were calculated, which ranged between 0.32: 0.48 for each primer. H0 was also calculated and the values ​​ranged between 0.32: 0.50. Results in the present study showed the effectiveness of EMS to induce in vitro mutation of Gardenia and this way can be used to develop breeding programs for ornamental plants. However, the use of more than one molecular marker in this type of studies may be more useful to cover multiple regions of the genome.
 

DOI

10.21608/asejaiqjsae.2022.240155

Keywords

Gardenia, Micropropagation - E.M.S - in vitro mutation - SSR markers

Authors

First Name

Mai

Last Name

Ebrahim

MiddleName

Ahmed

Affiliation

The Agricultural Research Center, Horticulture Research Institute,Ornamental Plants Research Department, Alexandria, Egypt.

Email

maielnawasany10289@gmail.com

City

Alexandri

Orcid

-

First Name

Naglaa

Last Name

Mohamed Esmaiel

MiddleName

-

Affiliation

The Agricultural Research Center, Horticulture Research Institute, Ornamental Plants Research Department, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

Ibrahim Abido

MiddleName

-

Affiliation

Faculty of Agriculture, Saba Basha, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Kadry Gaber

MiddleName

-

Affiliation

Faculty of Agriculture, Saba Basha, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

Volume

43

Article Issue

2

Related Issue

32901

Issue Date

2022-04-01

Receive Date

2022-04-15

Publish Date

2022-04-01

Page Start

261

Page End

269

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 43_Issue 2_Pages 261-269.pdf

PDF . 462.0kB

Link

https://asejaiqjsae.journals.ekb.eg/article_240155.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=240155

Order

9

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Regeneration, in Vitro Mutation and Evaluation of Genetic Stability of Gardenia Somaclones Via Ssr Markers

Details

Type

Article

Created At

22 Jan 2023