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223119

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Plant pest management

Abstract

Mitochondria sustain healthy brain function. Herein we aimed to evaluate the thiamethoxam (MX) effect on the rat brain mitochondria in addition to the protective role of N-acetylcysteine (NAC) against MX harmful effects. Thiamethoxam was administered orally with five doses each week for 28 days to male albino rats at 1/50 of the LD50 (31.26 mg/kg bw). The results demonstrated that the   thiamethoxam neurotoxicity was confirmed by the significant rising in acetylcholinesterase, and lactate dehydrogenase activities of plasma. A significant increase in mitochondrial antioxidants as superoxide dismutase and reduced glutathione was found. Also, significant induction of the oxidative stress and DNA damage via rising the malondialdehyde, and 8-hydroxy-2'-deoxyguanosine biomarkers was recorded by 32.5% and 118.61% respectively. Substantial depression in mitochondrial NADH dehydrogenase, cytochrome c reductase, cytochrome c oxidase, and Mg2+ ATPase complexesas well as 23 % cerebral infarction was manifested by histological evaluation using the dehydrogenase activity indicator, 2, 3, 5-triphenyl tetrazolium chloride staining. In conclusion, MX can pose a hazard to the integrity and functioning of rats' brain mitochondria, perhaps leading to neurodegenerative disorders. Additionally, earlier treatment of the synthetic antioxidant N-acetylcysteine could prove beneficial in combating the harmful effects of thiamethoxam.
 

DOI

10.21608/asejaiqjsae.2022.223119

Keywords

Brain, NADH dehydrogenase, Cytochrome c, DNA damage, neonicotinoid

Authors

First Name

Reda

Last Name

Abdel-Razik

MiddleName

Khamis

Affiliation

Pesticides Toxicity to Mammals and Aquatic Organisms, Central Agricultural Pesticide Lab., Agricultural Research Center, Alexandria, Egypt

Email

redarazik216@gmail.com

City

Alexandria

Orcid

0000-0002-0003-744X

First Name

Eman

Last Name

Mosallam

MiddleName

Mohamad

Affiliation

Mammalian & Aquatic Toxicology Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Alexandria, Egypt

Email

eman_mosallem2006@yahoo.com

City

Alexandria, Egypt

Orcid

-

First Name

Nadia

Last Name

Hamed

MiddleName

A

Affiliation

Mammalian & Aquatic Toxicology Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 21616 -El-Sabahia, Alexandria, Egypt

Email

nadia01056@gmail.com

City

Alexandria, Egypt

Orcid

-

Volume

43

Article Issue

1

Related Issue

30186

Issue Date

2022-03-01

Receive Date

2022-02-05

Publish Date

2022-03-30

Page Start

91

Page End

106

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 43_Issue 1_Pages 91-106.pdf

PDF . 525.0kB

Link

https://asejaiqjsae.journals.ekb.eg/article_223119.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=223119

Order

9

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Details

Type

Article

Created At

22 Jan 2023