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196416

Effect of Abscisic Acid, Salicylic Acid, Potassium Silicate, and Trichoderma harzianum As Biocontrol Agent to Induce the Tomato Resistance Against Early Blight Disease Caused by A

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Crop genetics and breeding

Abstract

In the present study and under greenhouse conditions, tomato plants cv. Albium, grown in potted soil inoculated with the early blight fungus A. solani, were treated with abscisic acid (ABA), salicylic acid (SA), and potassium silicate (PS) abiotic inducers, singly, and in combination with the biotic inducer Trichoderma harzianum. The different tested inducers significantly decreased the intensity (severity) of early blight disease on tomato, as well as some growth characteristics, photosynthetic capacity, defense-related enzymes and expression analysis of the PR1, PR3 and PR5 genes using Real Time-q PCR. PS+ Trichoderma showedthe lowest diseaseintensity, followed by ABA+ Trichoderma, and Trichoderma treatment had the highest disease intensity when compared to untreated inoculated control. PS + Trichoderma, ABA+ Trichoderma, and SA+ Trichoderma were also the most effective for the best growth characteristics (fresh shoot, dry shoot, fresh root, dry root weight, and total fruits weight/plant).  Photosynthetic pigment content was significantly increased with all treatments by chemical inducer and T. harzianum compared with A. solani treatment. PS + Trichoderma was the most effective and significantly enhanced chlorophyll "A" and chlorophyll "B"content comparing with the A. solani treatment, while, the highest level of carotene content was observed with alt+ABA+ Trichoderma treatment at 2days post inoculation. Peroxidase (POD), polyphenol oxidase (PPO) and Phenylalanine Ammonia-Lyase (PAL) enzymes activity and PR1, PR2 and PR5 gene expressions were significantly increased in all treatments with chemical inducer comparing with the treatment by A.solani, In addition to  the treatment by chemical inducer combined with Trichoderma were the most effective compared with chemical inducer alone. The maximum POD, PAL activities and PR1gene expression were observed with alt+PS + Trichoderma followed by alt+ABA+ Trichoderma while, PR2, PR5 gene expressions and POD activity reached the highest level with alt+PS+ Trichoderma followed by alt+SA+ Trichoderma and alt+ABA+ Trichoderma. Trichoderma was of the highest rate of the number of cfu in treatment with alt+PS+ Trichoderma with cfu was 5.9 x10, while the number of cfu in treatment with alt+ABA + Trichoderma was 3.77 x107 and the treatment with alt+SA + Trichoderma was 3.68 x107.

DOI

10.21608/asejaiqjsae.2021.196416

Keywords

Tomato, early blight, Alternaria solani, Trichoderma harzianum, Chemical inducer, Enzymes, gene expressions

Authors

First Name

Ibrahim

Last Name

Adss

MiddleName

Ahmed

Affiliation

Division of Genetics, Faculty of Agriculture, Damanhur University,

Email

adssibrahim@agr.dmu.edu.eg

City

Damanhour

Orcid

-

First Name

Ghoname. M.

Last Name

Amer

MiddleName

-

Affiliation

Division of Plant pathology, Faculty of Agriculture, Damanhur University, Al-Beheira, Egypt

Email

-

City

-

Orcid

-

First Name

Shokry R.

Last Name

Bayoumy

MiddleName

-

Affiliation

Division of Genetics, Faculty of Agriculture, Damanhur University, Al-Beheira, Egypt

Email

-

City

-

Orcid

-

First Name

Asia.

Last Name

R. Eid

MiddleName

-

Affiliation

Division of Plant pathology, Faculty of Agriculture, Damanhur University, Al-Beheira, Egypt

Email

-

City

-

Orcid

-

Volume

42

Article Issue

3

Related Issue

26297

Issue Date

2021-09-01

Receive Date

2021-08-20

Publish Date

2021-09-30

Page Start

773

Page End

788

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 42_Issue 3_Pages 773-788.pdf

PDF . 500.2kB

Link

https://asejaiqjsae.journals.ekb.eg/article_196416.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=196416

Order

16

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Effect of Abscisic Acid, Salicylic Acid, Potassium Silicate, and Trichoderma harzianum As Biocontrol Agent to Induce the Tomato Resistance Against Early Blight Disease Caused by Alternaria solani

Details

Type

Article

Created At

22 Jan 2023