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186822

Assessment of Egyptian Maize Hybrids for Their Capability of Plant Regeneration using Immature Embryo Culture

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Crop genetics and breeding

Abstract

The aim of this research was to determine the regeneration capability of five maize hybrids (Syngenta hybrid, Three-way hybrid (324), Single cross hybrid P344 (Pioneer), Single cross hybrid 30N 11 (Pioneer) and Three-way hybrid (321)) using immature embryo explants with four medium protocols. The analysis of variance for in vitro culture traits of explant response indicated that callus induction, callus weight and embryogenic callus significantly responded to maize genotypes, medium protocols and their interaction. Also differences among maize hybrids were highly significant for shoot formation (%), while they were not significant for root formation (%). The four medium protocols indicated highly significant differences for root formation (%) and shoot formation (%). Three-way hybrid (324) and Single cross hybrid 30 N11(Pioneer) had the highest induction frequency of shoot formation on medium protocol D. Both maizehybrids on medium protocol D could be successfully utilized for the in vitro selection studies for maize improvement.

DOI

10.21608/asejaiqjsae.2021.186822

Keywords

Maize, Immature embryo, tissue culture, Plant regeneration

Authors

First Name

Shaimaa M.

Last Name

Awad-Allah

MiddleName

-

Affiliation

Biotechnology Laboratory, Crop Science Department,Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Yohana

Last Name

Kuku

MiddleName

-

Affiliation

Biotechnology Laboratory, Crop Science Department,Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Sanaa I.

Last Name

Milad

MiddleName

-

Affiliation

Biotechnology Laboratory, Crop Science Department,Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

M. N.

Last Name

Barakat

MiddleName

-

Affiliation

Biotechnology Laboratory, Crop Science Department,Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt

Email

mnrbarakat@yahoo.com

City

-

Orcid

-

Volume

42

Article Issue

3

Related Issue

26297

Issue Date

2021-09-01

Receive Date

2021-06-05

Publish Date

2021-09-30

Page Start

619

Page End

627

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 42_Issue 3_Pages 619-627.pdf

PDF . 396.3kB

Link

https://asejaiqjsae.journals.ekb.eg/article_186822.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=186822

Order

4

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Assessment of Egyptian Maize Hybrids for Their Capability of Plant Regeneration using Immature Embryo Culture

Details

Type

Article

Created At

22 Jan 2023