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159990

In Vitro Propagation and Mutagenesis of Jatropha Curcas

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Last updated: 01 Jan 2025

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Horticulture

Abstract

This study was carried out in the Tissue Culture Laboratory, Department of Flowers, Ornamental Plants and Landscape Gardens, Faculty of Agriculture (El-Shatby), Alexandria University, Egypt during the period from 2012 to 2015 to establish an efficient and reliable protocol for in vitro propagation and improve of Jatrophacurcas. Seeds of Jatropha were effectiveness of mutation induction by different ways. Planted on Murashige and Skoog media (MS), using different combinations Indol acetic acid (IAA) at 0, 1 and 2 mg/l and benzyl adenine (BA) at 0, 1, 2 and 3 mg/l of  kintin (Kin)  at 2.0 mg/l and for shoot regeneration .While the media used for rooting was amendmented with different (IAA) at 0, 1 and 2 mg/l and indol butyric acid (IBA) at 0, 1, 2 and 3 mg/l. Oil was extracted from seeds and callus formed on MS medium containing combinations of BA at 0.5, 1 and 1.5 mg/l,(NAA) at 1, 2 and 3 mg/l and 2,4-dichlorophenoxyacetic acide (2,4-D)  at 0.5 mg/l.
Regenerated rooted plants were then treated by colchicines or glufosinate ammonium at 1, 2, 3 and 4 mg/l for each. The best medium for shoot regeneration was MS amended with BA at 2 and 3 mg / l and IAA at 1mg/l , while the best rooting medium was MS amended with IAA at 2mg/l and IBA at1 and 2mg/l. For obtaining the highest percentage of oil content from   callus, medium at 0.5 mg/l BA combined with 2 mg/l NAA and 0.5 mg/l 2,4-D gave the best results. For the induction of genetic variation glyphosinate ammonium to be added at 3 mg/l, while colchicines had at all concatenation no effect on cell division of Jatropha curcas plant.

DOI

10.21608/asejaiqjsae.2021.159990

Keywords

In vitro culture, Jatropha curcas, Micro propagation, Colchicine - Glufosinate ammonium - RABD

Authors

First Name

M.G.

Last Name

EL-Torky

MiddleName

-

Affiliation

Floriculture,Ornamental Horticulture and Landscape Gardening, Fac.Agric., Alex.Univ.,Egypt.

Email

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City

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Orcid

-

First Name

H.M.

Last Name

EL-Naggar

MiddleName

-

Affiliation

Floriculture,Ornamental Horticulture and Landscape Gardening, Fac.Agric., Alex.Univ.,Egypt

Email

-

City

-

Orcid

-

First Name

N.A.

Last Name

El-Shanhorey

MiddleName

-

Affiliation

Department of Botanical Garden) Antoniades), Horticultural Research Institute, (A.R.C), Alex, Egypt.

Email

-

City

-

Orcid

-

First Name

A.A.

Last Name

yousef

MiddleName

-

Affiliation

Department of Botanical Garden) Antoniades), Horticultural Research Institute, (A.R.C), Alex, Egypt.

Email

-

City

-

Orcid

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Volume

42

Article Issue

JANUARY- MARCH

Related Issue

20854

Issue Date

2021-01-01

Receive Date

2021-02-19

Publish Date

2021-03-30

Page Start

209

Page End

222

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 42_Issue JANUARY- MARCH_Pages 209-222.pdf

PDF . 943.9kB

Link

https://asejaiqjsae.journals.ekb.eg/article_159990.html

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https://asejaiqjsae.journals.ekb.eg/service?article_code=159990

Order

20

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

In Vitro Propagation and Mutagenesis of Jatropha Curcas

Details

Type

Article

Created At

22 Jan 2023