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84490

Morphological Characterization and Pathogenicity of pythium sp. Infecting Cucumber Seedlings in Greenhouses

Article

Last updated: 01 Jan 2025

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Tags

Plant diseases

Abstract

Greenhouse-grown cucumber seedlings exhibiting typical symptoms of Pythium damping-off and root rot were collected. The causal agents were isolate on Pythium selective medium; nystatin, ampicillin, rifampicin, and miconazole (NARM) in potato dextrose agar (PDA). Four different isolates was isolated and their pathogenicity was tested using cotyledon and young seedlings assays. Results of these isolates showed variation in their ability to infect cucumber seedling. Isolate "GZ-11" was highly pathogenic, caused 100 % pre- and post-emergence damping-off. The isolate was identified to the species level based on morphological characters along with sequences of the internal transcribed spacer (ITS) of the ribosomal DNA and showed 99% similarity to Pythium spinosum (Synonym of Globisporangium spinosum). Aggressiveness of P. spinosum was investigated at two levels of inoculum concentrations (10 and 20 g/kg soil). Results reported that P. spinosum was highly aggressive and significantly reduced cucumber seed germination compared to the control.
 

DOI

10.21608/asejaiqjsae.2020.84490

Keywords

Pythium, pathogenicity, Cucumber, ITS and phylogenetic treev

Authors

First Name

Mohamed M.

Last Name

Gharieb

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Menoufia University, Egypt.

Email

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City

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Orcid

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First Name

Gaber A.

Last Name

Abo-Zaid

MiddleName

-

Affiliation

Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934, Egypt.

Email

-

City

-

Orcid

-

First Name

Shimaa I.

Last Name

Bashir

MiddleName

-

Affiliation

plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934, Egypt.

Email

y_basher@yahoo.com

City

-

Orcid

-

First Name

Elsayed E.

Last Name

Hafez

MiddleName

-

Affiliation

3Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934, Egypt.

Email

-

City

-

Orcid

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Volume

41

Article Issue

JANUARY- MARCH

Related Issue

10673

Issue Date

2020-03-01

Receive Date

2020-02-25

Publish Date

2020-03-31

Page Start

123

Page End

128

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 41_Issue JANUARY- MARCH_Pages 123-128.pdf

PDF . 378.2kB

Link

https://asejaiqjsae.journals.ekb.eg/article_84490.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=84490

Order

12

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Morphological Characterization and Pathogenicity of pythium sp. Infecting Cucumber Seedlings in Greenhouses

Details

Type

Article

Created At

22 Jan 2023