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36518

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Food Science and technology

Abstract

Three kinds of processed meat (Luncheon, Burger , Minced meat) were examined for the presence of Listeria species.Collected samples purchased from local supermarkets were from three different Egyptian companies. All the  bacterial isolates were cultured on specific media after pre-enrichment and enrichment cultures. The colonial morphology depends strongly up on the media used and the incubation conditions provided.  (Oxford Listeria Agar Base)was used as specific medium for Listeria. The bacterial  distribution of Listeria was, 2.22% in Beef burger, 13.6%. in luncheon and there was no bacterial growth in minced meat. In this study;  gram staining., morphological characterization, molecular identification using 16S rDNA  partial sequencing  and specific gene Iap gene  of Listeria were carried out.

DOI

10.21608/asejaiqjsae.2019.36518

Keywords

Listeria Species, specichic PCR

Authors

First Name

Mona M.

Last Name

Hassan

MiddleName

-

Affiliation

Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt

Email

mona.mekhemr@yahoo.com

City

-

Orcid

-

First Name

Samia A.

Last Name

AbdAl-Aziz

MiddleName

-

Affiliation

Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt

Email

samiaabdelaziz1@yahoo.com

City

-

Orcid

-

First Name

Asmaa M.

Last Name

Megahed

MiddleName

-

Affiliation

Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Hagar A.

Last Name

Abdel Rassoul

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Yasser M.

Last Name

Mabrouk

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

40

Article Issue

APRIL- JUNE

Related Issue

5258

Issue Date

2019-04-01

Receive Date

2019-05-27

Publish Date

2019-06-30

Page Start

333

Page End

339

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 40_Issue APRIL- JUNE_Pages 333-339.pdf

PDF . 415.7kB

Link

https://asejaiqjsae.journals.ekb.eg/article_36518.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=36518

Order

11

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Details

Type

Article

Created At

22 Jan 2023