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Expression Profile of Wheat DNA Methyltransferases Genes in Egyptian Wheat (Triticum Aestivum) Varieties Under PEG Induced Dehydration

Article

Last updated: 01 Jan 2025

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Tags

Crop genetics and breeding

Abstract

DNA methyltransferases are group of enzymes that add methyl group to cytosine in DNAs. Methylation regulates gene expression under normal conditions as well as in response to adverse conditions. DNA methyltransferases gene expression level was estimated in seven Egyptian wheat varieties; (Misr1, Giza168, Sakha94, Sids1, Gemmiza7, Gemmiza11, and Shandawel1) under drought induced by polyethylene glycol (PEG) using semiquantitative PCR of cDNA generated from total RNA. The seven tested varieties showed variations in four wheat DNA methyltransferase (Met) gene expression. Sids1 variety showed high expression level of Met1, Met2b, CMT, and Met3. The other varieties varied in Met gene expression levels. Met1 gene expression ranged from 66 to 47% of Sids1 gene expression, Met2b was 71 to 44% of Sids1 gene expression, CMT level ranged from 60 to 22% Sids1 gene expression Met3 expression was 78-21% of Sids1 gene expression. The results concluded that, in sidsiratety wheat Mets activities responde faster to PEG-induced dehydration. Also, variation in Mets genes expression may be due to resistance variation of resistance of wheat varieties to abiotic stresses, such as heat, salinity and drought. Consequently, Met genes activities could be used in the evaluation of new wheat varieties and genetic manipulation of wheat resistance to biotic and abiotic stresses.

DOI

10.21608/asejaiqjsae.2018.21876

Keywords

Gene expression, abiotic stress, wheat resistance

Authors

First Name

Mona M.

Last Name

Elseehy

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Alexandria University

Email

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City

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Orcid

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First Name

Ahmed M.

Last Name

El-Shehawi

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Alexandria University Department of Biotechnology, Taif University, Taif, Saudi Arabia

Email

-

City

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Orcid

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Volume

39

Article Issue

OCTOBER- DECEMBER

Related Issue

3878

Issue Date

2018-12-01

Receive Date

2018-11-15

Publish Date

2018-12-30

Page Start

695

Page End

701

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 39_Issue OCTOBER- DECEMBER_Pages 695-701.pdf

PDF . 315.3kB

Link

https://asejaiqjsae.journals.ekb.eg/article_21876.html

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https://asejaiqjsae.journals.ekb.eg/service?article_code=21876

Order

15

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Expression Profile of Wheat DNA Methyltransferases Genes in Egyptian Wheat (Triticum Aestivum) Varieties Under PEG Induced Dehydration

Details

Type

Article

Created At

22 Jan 2023