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16883

Molecular Detection and Pathogenic Capability of Agrobacterium tumefaciens Isolates from Olive

Article

Last updated: 01 Jan 2025

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Tags

Plant diseases

Abstract

Crown gall, caused by Agrobacterium tumefaciens, first reported on olive in Libya. A partial 16SrRNA gene sequence confirmed the identity of the A. tumefaciens bacterial isolates. The Ti-plasmid (induce tumors formation) is the real reason of their pathogenicity. Meanwhile, the prerequisite for the control of A. tumefaciens is to have a rapid and reliable detection method of pathogenic isolates in order to select healthy plants therefore, we detected two plasmidic genes, virD2 gene of the virulence (vir) region (amplified by virD2A/C and virD2A/E primers), and T-DNA (on tms gene). The PCR amplified bands analysis had a correspondence to the pathogenicity assay (carrot tumorgenicity) and the biochemical determents' of the isolates genus.

DOI

10.21608/asejaiqjsae.2018.16883

Keywords

Agrobacterium tumefaciens, PCR, Ti-plasmid, virD2, pathogenicity

Authors

First Name

Nagia M.

Last Name

Jadalla

MiddleName

-

Affiliation

Plant protection Department, Faculty of Agriculture -Omar Al-mukhtar University. Elbida-Libya

Email

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City

-

Orcid

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First Name

Said I.

Last Name

Behiry

MiddleName

-

Affiliation

Agricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed A.M.

Last Name

Adam

MiddleName

-

Affiliation

Plant protection Department, Faculty of Agriculture -Omar Al-mukhtar University. Elbida-Libya

Email

-

City

-

Orcid

-

Volume

39

Article Issue

July-September

Related Issue

1814

Issue Date

2018-07-01

Receive Date

2018-09-06

Publish Date

2018-09-30

Page Start

521

Page End

526

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 39_Issue July-September_Pages 521-526.pdf

PDF . 294.1kB

Link

https://asejaiqjsae.journals.ekb.eg/article_16883.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=16883

Order

17

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Molecular Detection and Pathogenic Capability of Agrobacterium tumefaciens Isolates from Olive

Details

Type

Article

Created At

22 Jan 2023