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15649

Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions

Article

Last updated: 01 Jan 2025

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-

Tags

Crop genetics and breeding

Abstract

Cyst nematodes involve over than 90 known species into six genera, G. rostochiensis and G. pallida are most important pathogens of potato crops where their economically a yield losses. The aim of present investigation is characterization the cyst nematode species (G. rostochiensis) using molecular techniques during plant-nematode interaction. The molecular identification of cyst nematode was carried out using ITS PCR. The results of ITS-PCR showed two bands with different molecular sizes 238 bp and 370 bp. The metabolic activation of potato leaves infected with cyst nematodes was detected. The results confirmed that, G.rostochiensis significantly elevated the contents of shoots lipid peroxidation (MDA) as compared to the untreated healthy plants.
 

DOI

10.21608/asejaiqjsae.2018.15649

Keywords

Cyst nematodes, ITS PCR, potato

Authors

First Name

Dina H.

Last Name

Elkobrosy

MiddleName

-

Affiliation

Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934 Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Dalia G.

Last Name

Aseel

MiddleName

-

Affiliation

Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934 Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Nader R.

Last Name

Abdelsalam

MiddleName

-

Affiliation

Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria,Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed A.

Last Name

El-Saedy

MiddleName

-

Affiliation

Plant Pathology Department, Faculty of Agriculture (Elshatby),Alexandria University, 21545 Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Saad

Last Name

Shama

MiddleName

-

Affiliation

Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Elsayed E.

Last Name

Hafez

MiddleName

-

Affiliation

Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934 Alexandria, Egypt.

Email

-

City

-

Orcid

-

Volume

39

Article Issue

July-September

Related Issue

1814

Issue Date

2018-07-01

Receive Date

2018-09-05

Publish Date

2018-09-30

Page Start

478

Page End

481

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 39_Issue July-September_Pages 478-481.pdf

PDF . 206.3kB

Link

https://asejaiqjsae.journals.ekb.eg/article_15649.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=15649

Order

12

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions

Details

Type

Article

Created At

22 Jan 2023