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Survey of Bacteria in the Farm of Faculty of Agriculture ,Al-Azahr University, Assiut Governorate,Egypt

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Last updated: 01 Jan 2025

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Tags

Aquatic and terrestrial environment

Abstract

Two hundred and fifteen  soil bacteria isolates able to reduce NO-3, 210 produced nitrous oxide (N2O), even  only 47 were respiratory denitrifiers  . Nitrite or NH+4 was the major product of NO-3 reduction by the non-denitrifying organisms, but typically about 5 to 10% and up to 34% of the NO-3  reduced by them was released as N2O during a 2- week incubation period. Bacillus and Enterobacter were the most commonly observed genera of non-denitrifying N2O producers. Fermentative NO-2reduction and N2O production by a Bacillus sp. and a Citrobacter sp. were characterized in pure culture studies. Dinitrogen (N2) was not produced in detectable quantities by these organisms. When added to autoclaved soil, they accumulated more N2O than two denitrifying pseudomonads, since the latter consume and produce N2O. In tryptic soy broth (TSB), which allows active fermentative growth, NH+4 was apparently the major product of NO-3 reduction.  Added NH+4 did not inhibit N2O production or apparent reduction to NH+4, indicating that these processes are not essimilatory  . The effect added glucose on N2O production varied with the organism and media composition. Nitrous oxide production from NO-2 by these organisms was shown to be at least partially a biochemical reaction. The N2O evolved slowly in bath cultures and mostly after apparent growth ceased. This is apparently a novel mechanism of N2O generation which differs significantly from respiratory denitrification .
 

DOI

10.21608/asejaiqjsae.2017.4883

Keywords

Nutrient broth (NB), tryptie soy broth (TSB), nitrite reduction, denitrification, dissimilatory ammonium production

Authors

First Name

Said A.M.

Last Name

El-Sayed

MiddleName

-

Affiliation

Soil and Water Science Dept.,Faculty of Agric. In Assiut, Al –Azhar University

Email

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City

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Orcid

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Volume

38

Article Issue

October-December

Related Issue

694

Issue Date

2017-10-01

Receive Date

2017-12-07

Publish Date

2017-12-31

Page Start

956

Page End

966

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 38_Issue October-December_Pages 956-966.pdf

PDF . 309.9kB

Link

https://asejaiqjsae.journals.ekb.eg/article_4883.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=4883

Order

30

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Survey of Bacteria in the Farm of Faculty of Agriculture ,Al-Azahr University, Assiut Governorate,Egypt

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Article

Created At

22 Jan 2023