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4184

Physico-Chemical Poperties of Chromatin Isolated from Vicia Faba Embryonic Axes

Article

Last updated: 01 Jan 2025

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Subjects

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Tags

Crop genetics and breeding

Abstract

Faba bean (Vicia faba L.), an old-world grain legume, is grown approximately in 3 m/ha area world-wide from temperate. In favorable conditions, it gives very high yields. The present work was carries out to produce a good preparation of chromatin isolated from embryonic axes of Vicia faba, Chromatin which can be isolated as a chemically defined entity including DNA, RNA, histone and non- histone protein. Vicia faba variety white and Brassica oleracea variety botrytis as two unrelated species.
The activity of chromatin can be also measured by its stabilization degree against thermal denaturation. Chromatin in 1 M NaCl was purified by chromatography on Sephadex G- 25 Fine/ Pharmacia fine chemicals AB, Sweden. The purity of chromatin prepartions was estimated, Extraction of histones was carried out from purified chromatin with 0.4 HCl by using 10 ml chromatin in each analysis.
The products of genetic activity vary greatly in amount in different cell types and in the same cell types and in the same cell type at successive stages of differentiation
 

DOI

10.21608/asejaiqjsae.2017.4184

Keywords

Vicia faba, Faba bean, Chromatin Isolated, composition of chromatin

Authors

First Name

Azza M.

Last Name

Morgan

MiddleName

-

Affiliation

Dept. Genetics Fac- Agric. Alexandria Uni. Alexandria, Egypt.

Email

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City

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Orcid

-

First Name

Mohamed.A

Last Name

Seehy

MiddleName

-

Affiliation

Dept. Genetics Fac- Agric. Alexandria Uni. Alexandria, Egypt.

Email

-

City

-

Orcid

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Volume

38

Article Issue

October-December

Related Issue

694

Issue Date

2017-10-01

Receive Date

2017-10-03

Publish Date

2017-10-01

Page Start

746

Page End

751

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 38_Issue October-December_Pages 746-751.pdf

PDF . 129.9kB

Link

https://asejaiqjsae.journals.ekb.eg/article_4184.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=4184

Order

10

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Physico-Chemical Poperties of Chromatin Isolated from Vicia Faba Embryonic Axes

Details

Type

Article

Created At

22 Jan 2023