The present research was carried out in Sabahia Agricultural Research Station, Alexandria, Egypt, (2010 - 2013) to study root and hypocotyl colors traits in sugar beet in F2 plants derived from hybridization among sugar beet multigerm diploid American inbred line (C39) and Egyptian table beet. In addition to, Gene frequency of hypocotyl colors (Red, Pink and green) in the three multigerm sugar beet populations introduced from USA "3859 (Sp), Z120 and 3915 (Sp)". Results revealed that F2 phenotype seedlings from (C39 X Table beets) were segregated to three groups according to leaf, hypocotyl and root colors. The main synopsis are gene frequency of hypocotyl colors in the three examined populations indicated that green hypocotyl (rr) were presented in rear percentages (eg, 0.016, 0.01 and 0.02) in populations "3859 (Sp), Z120 and 3915 (Sp)" in the three examined populations, respectively.