This investigation was conducted during three seasons 2010, 2011 and 2012 on Zaghloul date palm cultivar grown in calcareous soil at AL- Nahda region near Alexandria governorate, Egypt. The palms received the recommended N dose of each mineral fertilizer only and or from organic N and bio fertilizers to study the effect of such treatments on date palm yield, fruit nitrate and nitrite content. The obtained results clarified that all used treatments of organic and bio fertilizers were effective in improving date palm yield and decreased fruit content of No3 and No2 in comparison with 100% mineral N. It was also noticed that combined treatment of 50%mineral N+50%organic manure with bio fertilizer is the superior treatment for increasing the values of yield and decreasing its content of No3 and No2. Hence, it could be concluded that, minimizing the use of chemical nitrogen fertilizer to half of recommended dose through addition of 50% mineral N+ 50% organic manure with bio fertilizer and also, the utilization of organic and bio fertilizers is considered as a promising alternative for chemical fertilizers to avoid pollution and reduce costs.