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2936

Efficiency of Using Some New Insecticides against Cotton Leaf Worm (Spodopteralittoralis) Based on Biochemical and Molecular Markers

Article

Last updated: 01 Jan 2025

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Tags

Crop genetics and breeding

Abstract

Bioassays were carried out for evaluating the toxicity of different insecticides against the susceptible strain of cotton Leafworm, Spodoptera littoralis (Boisd.). Toxicity data based on LC50 values indicated that the proclam® is more toxic insecticide against the laboratory strain, while, the OP syclon®was less toxic. Esterase isozymes exhibited a wide range of variability among the pupae and larvae. Two common bands were detected among all samples in pupae and larvae. Proclam showed one band in pupae
(Es.C2) compared with other treatments and in larvae detected one band in (Es.C3) and detecte the highest band number (6 bands) comparing with other insectcides. The results of biochemical analysis showed that there is arelationship between the resistance to insecticide and the increase of enzyme activity such as esterase enzyme. Five out of ten oligonucleotide primers (50%) used in the
RAPD analysis gave unique markers. Out of the five primers, two detected single unique markers (OPA-02 and OPA-15), two gave two markers (OPA-05 and opc-02), and one yielded three unique markers (OPC-12).Additionally, compared with the control worms, cotton leaf worm exposed to insecticides recorded the emergence of 7 new fragments from 98 total fragments. So, the total number of fragments which disappeared in response to both treatments was 98 fragments. Exposure to insecticides at all concentrations revealed the increase or decrease the number of amplified polymorphic DNA and increased gene expression in comparison to control cotton leaf worm.

DOI

10.21608/asejaiqjsae.2015.2936

Keywords

Cotton leaf worm, Spodopter alittoralis, Bioassay, esterase Isozymes and RAPD

Authors

First Name

Heidi A.

Last Name

Ali

MiddleName

-

Affiliation

PlantProtection Research Institute,Agric. Res. Center, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed M.

Last Name

Kordy

MiddleName

-

Affiliation

Plant Protection Department, Faculty of Agriculture (SabaBasha), Alexandria University, Egypt;

Email

-

City

-

Orcid

-

First Name

Ahmed E.

Last Name

Khaled

MiddleName

-

Affiliation

Agricultural Botany Department, Faculty of Agriculture (Saba-Basha), Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Nabeill A.

Last Name

Hassan

MiddleName

-

Affiliation

PlantProtection Research Institute,Agric. Res. Center, Egypt;

Email

-

City

-

Orcid

-

First Name

Nader

Last Name

R. Abdelsalam

MiddleName

-

Affiliation

Agricultural Botany Department, Faculty of Agriculture Saba-Bacha, Alexandria University, 22 Tag El-Roasa St. Saba Pacha, Boulkly P.O. Box: 21531.

Email

nader.wheat@yahoo.com

City

-

Orcid

-

Volume

36

Article Issue

OCTOBER- DECEMBER

Related Issue

531

Issue Date

2015-12-01

Receive Date

2015-10-26

Publish Date

2015-12-30

Page Start

303

Page End

313

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 36_Issue OCTOBER- DECEMBER_Pages 303-313.pdf

PDF . 7.2MB

Link

https://asejaiqjsae.journals.ekb.eg/article_2936.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2936

Order

3

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Efficiency of Using Some New Insecticides against Cotton Leaf Worm (Spodopteralittoralis) Based on Biochemical and Molecular Markers

Details

Type

Article

Created At

22 Jan 2023