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2881

Gene Expression of Wheat (Triticum aestivum) Aquaporins under PEG-Induced Dehydration

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Crop genetics and breeding

Abstract

Plant aquaporin genes regulate water movement through cellular membranes and determine the plant water relationship and plant adaptation to different environmental stresses. The expression of three PIP genes and one TIP gene was studied in response to PEG-induced dehydration in four Egyptian commercial wheat varieties. Semiquantitative estimation of RNA level via cDNA synthesis and PCR was used. Results showed that variations among the four studied genes in wheat varieties under normal condition and in the presence of PEG. Under normal conditions, Misr1 exhibited the highest level of PIP1 expression, Sids1 exhibited the highest level of PIP2 and PIP3 expression, and Gemmiza7 exhibited the highest expression of TIP1. Under dehydration induced by PEG, all three PIP genes (PIP1, PIP2, PIP3) showed various levels of transcription downregulation. PIP1 gave the highest level of downregulation in all varieties. Sids1 showed the highest response to PEG-induced dehydration because under normal conditions it exhibited high PIP expression and low TIP expression, whereas under dehydration condition it showed high downregulation of PIP genes and high upregulation of the TIP gene. This would be related to its high adaptation to dehydration. Results of this study will enhance our understanding of plant aquaporin expression profile and utilizing this in improvement of plant tolerance to dehydration. 

DOI

10.21608/asejaiqjsae.2015.2881

Keywords

Wheat, aquaporins, Gene expression, Dehydration, PCR

Authors

First Name

Mona M.

Last Name

Elseehy

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt.

Email

monaahmedma@yahoo.com

City

-

Orcid

-

First Name

Ahmed M.

Last Name

El-Shehawi

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt. Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia.

Email

-

City

-

Orcid

-

Volume

36

Article Issue

April-June

Related Issue

529

Issue Date

2015-06-01

Receive Date

2015-06-04

Publish Date

2015-06-30

Page Start

180

Page End

187

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 36_Issue April-June_Pages 180-187.pdf

PDF . 319.4kB

Link

https://asejaiqjsae.journals.ekb.eg/article_2881.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2881

Order

10

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Gene Expression of Wheat (Triticum aestivum) Aquaporins under PEG-Induced Dehydration

Details

Type

Article

Created At

22 Jan 2023