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2643

The Use of Biotechnology for Biodegradation of Paper Manufacturing Wastes (Kraft Lignin) Via Egyptian Bacterial Isolates

Article

Last updated: 01 Jan 2025

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Tags

Soil Fertility & plant nutrition

Abstract

Lignin contained in pulping liquor that is generated during the pulping process for papermaking is a disposal
problem for the pulp and paper industry because it causes a serious pollution and toxicity problem in aquatic
ecosystems, owing to its low biodegradability and high range of color. Four bacterial strains were isolated from soil, water and activated sludge samples which were collected from the effluent treatment plant of Rakta Pulp and Paper Company, Eltabia, Alexandria, Egypt in sterile test tubes. After isolation and purification of the bacterial isolates, they were tested for the degradation of kraft lignin (KL) using sterile mineral salt medium (MSM) containing KL 1000 mg l–1 (designated hereafter L-MSM) and
supplemented with 1.0% glucose and 0.5% peptone (w/v) added in L-MSM-broth and L-MSM-agar as growth
supportive substrates and incubated for seven days under aerobic conditions at 30 °C and 120 rpm. Samples were withdrawn periodically at 1-day intervals for seven days and analysed for bacterium growth, reduction of color and residual KL content. The isolates were subjected to 16S rDNA sequence for identification. Partial sequence of 16S rDNA revealed that these isolates were Bacillus subtilis subsp. Subtilis, Citrobacter farmeri, Escherichia fergusonii and Stenotrophomonas rhizophila. The average reduction of color was 85.4, 70.1, 61.7 and 74.5 % and lignin degradation 71.4, 62.2, 54.5 and 57.1%, respectively.

DOI

10.21608/asejaiqjsae.2011.2643

Keywords

Key words: Biotechnology, biodegradation, kraft lignin, 16S rDNA and bacteria

Authors

First Name

Abuo Gabal

Last Name

ashgan

MiddleName

A.

Affiliation

Faculty of Agriculture – Saba Basha, Genetic Department, Alexandria University, Egypt.

Email

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City

-

Orcid

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First Name

Abd Elsalam

Last Name

Hassan

MiddleName

E.

Affiliation

2Arid lands Cultivation and Development Research Institute, Soil and water Technologies Department, City for Scientific Research and Technology Applications, Egypt.

Email

-

City

-

Orcid

-

First Name

Abd Al-Aziz

Last Name

Samia

MiddleName

A.

Affiliation

Genetic Engineering and Biotechnology Research Institute, Nucleic Acids Research Department, City for Scientific Research and Technology Applications, Egypt.

Email

-

City

-

Orcid

-

First Name

El-Hanafy

Last Name

Amr

MiddleName

A.

Affiliation

Genetic Engineering and Biotechnology Research Institute, Nucleic Acids Research Department, City for Scientific Research and Technology Applications, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdallah

MiddleName

E.

Affiliation

2Arid lands Cultivation and Development Research Institute, Soil and water Technologies Department, City for Scientific Research and Technology Applications, Egypt.

Email

-

City

-

Orcid

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Volume

32

Article Issue

OCTOBER- DECEMBER

Related Issue

410

Issue Date

2011-12-01

Receive Date

2011-11-13

Publish Date

2011-12-30

Page Start

392

Page End

399

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 32_Issue OCTOBER- DECEMBER_Pages 392-399.pdf

PDF . 573.3kB

Link

https://asejaiqjsae.journals.ekb.eg/article_2643.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2643

Order

3

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

The Use of Biotechnology for Biodegradation of Paper Manufacturing Wastes (Kraft Lignin) Via Egyptian Bacterial Isolates

Details

Type

Article

Created At

22 Jan 2023