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2633

Protease and Lipase Activities of Pseudomonas Spp. Isolated from Pasteurized Milk

Article

Last updated: 22 Jan 2023

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Biotechnology

Abstract

Raw milk deteriorates in only a few days even when stored under refrigeration temperatures. Moreover,
pasteurized and refrigerated milk has a shelf life of 7 to 10 days. Psychrotrophic bacteria have been recognized as a recurring problem in refrigerated storage and distribution of perishable food products. This study was performed to isolate, characterize and evaluate the prevalence of Pseudomonas spp. in pasteurized milk. Thirty five pasteurized milk samples were collected aseptically from different dairy shops, supermarkets and groceries in different areas in Sadat city, Menuofiya Governorate, Egypt and analyzed for total viable count and Pseudomonas spp. occurrence. Pseudomonas aeruginosa found to be the predominant from the Pseudomonas spp. followed by Pseudomonas fluorescens. The total viable count ( cfu per ml) average ± SD were 3.50× 104 cfu /ml ± 0.085. Out of ten Pseudomonas species isolates were examined to its proteolytic and lipolytic activity, two isolates had proteolytic activity, while three had lipolytic activity and five isolates had both.

DOI

10.21608/asejaiqjsae.2011.2633

Keywords

Pseudomonas aeruginosa, Pseudomonas fluorescens, proteolytic and lipolytic activity

Authors

First Name

Hoda

Last Name

Mahrous

MiddleName

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Affiliation

Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, Menuofiya University, Egypt.

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Orcid

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Volume

32

Article Issue

JULY- SEPTEMBER

Related Issue

409

Issue Date

2011-09-01

Receive Date

2011-08-02

Publish Date

2011-09-30

Page Start

295

Page End

299

Print ISSN

1110-0176

Online ISSN

2536-9784

Link

https://asejaiqjsae.journals.ekb.eg/article_2633.html

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https://asejaiqjsae.journals.ekb.eg/service?article_code=2633

Order

3

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

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Article

Created At

22 Jan 2023