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Isolation, Phylogenetic Analysis and Screening of Biosurfactants Producing By Locally Isolated Bacteria

Article

Last updated: 01 Jan 2025

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Tags

Biotechnology

Abstract

Biosurfactants or surface-active compounds are produced by microoaganisms. These molecules reduce surface tension both aqueous solutions and hydrocarbon mixtures. 45 bacterial strains previously isolated from petrochemical wastes around Oil Refinery Company in Tanta, Al Ggharbiyah, Egypt. Selection  and screening of biosurfactant producer(s), via four different biioassays; (i) surface tension measurements, (ii) blood hemolysis test, (iii) drop-collapsing test, and (iv) emulsification test. Two isolates coded 3C.A and C1 respectively were chosen to be the best candidates for biosurfactant production. The highest production of biosurfactants produced by 3C.A isolate grown on medium supplemented with 3g/L ammonium sulfate as a nitrogen source and (4%) glucose as carbon source and incubated at 37°C. The biosurfactants were found capable of producing a relatively stable emulsion hydrocarbon at pH 7 and thermostable for 1 hour at 75 °C, based on the value of surface tension. The phylogenetic analysis based on 16S rDNA and physiological characzterization indicated that the isolates of strain 3C.A and C1 as well Bacillus subtilis Eg1 and pseudonamnas sp respectivly.

DOI

10.21608/asejaiqjsae.2011.2415

Keywords

Biosurfactant, Emulsification, Surface tension, phylogentic analysis

Authors

First Name

Abuo Gabble

Last Name

A. Ashgan

MiddleName

-

Affiliation

Faculty of Agriculture – Saba Basha Genetic Department Alexandria University, Egypt.

Email

ashgabal@yahoo.com

City

-

Orcid

-

First Name

Amer

Last Name

A. Ranya

MiddleName

-

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City for Scientific Research and Technology Application, Egypt.

Email

-

City

-

Orcid

-

First Name

Abd Elsalam

Last Name

E. Hassan

MiddleName

-

Affiliation

Arid lands Cultivation and Development Research Institute Soil and water Technologies Department (Soil Microbiology), City for Scientific Research and Technology Applications, Egypt. Tel: 002 0105420541, 0020 3 4593422, Fax: 0020 3 4593423

Email

-

City

-

Orcid

-

First Name

Teama

Last Name

E. Elsayed

MiddleName

-

Affiliation

Faculty of Agriculture – Saba Basha Genetic Department Alexandria University, Egypt.

Email

-

City

-

Orcid

-

Volume

32

Article Issue

APRIL- JUNE

Related Issue

408

Issue Date

2011-06-01

Receive Date

2011-06-13

Publish Date

2011-06-30

Page Start

182

Page End

192

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 32_Issue APRIL- JUNE_Pages 182-192.pdf

PDF . 546.8kB

Link

https://asejaiqjsae.journals.ekb.eg/article_2415.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2415

Order

10

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Isolation, Phylogenetic Analysis and Screening of Biosurfactants Producing By Locally Isolated Bacteria

Details

Type

Article

Created At

22 Jan 2023