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1878

Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti

Article

Last updated: 22 Jan 2023

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Tags

Crop genetics and breeding

Abstract

In order to establish an effective regeneration system of Stevia (Stevia rebaudiana) var. sponti, different concentrations of phytohormones were added to MS basal medium individually or in combination with (IAA, BAP and NAA) as callus induction media. The obtaining results indicated that MS basal medium which containing 0.5 mg/l of NAA and 0.5 BAP was the most efficient and effective medium for callus induction.
For shoot regeneration, different recombination of phytohormones were used, addition of NAA (0.1 mg/l) and BAP (1.0 mg/l) to MS basal medium under light condition caused high increase in shooted calli percentage.
Vigor root system was developed after the addition of 3 mg/l of IBA to MS basal medium, while the addition of high concentration of sucrose (80 g ∕ L) found to be ineffective factor for root regeneration and growth. Micropropagation system was performed by exposure of axillaries and terminal buds to solid MS medium which contained 0.1 IAA and 1.0 BA. Then, clusters were cut into small number of plantlets in MS liquid media without any hormone. This method was found to be more effective than using hormonal medium for micropropagation. 

DOI

10.21608/asejaiqjsae.2007.1878

Authors

First Name

Ouf

Last Name

A,A

MiddleName

-

Affiliation

Laboratory of Biotechnology. Sugar crops Res. Inst., Agric. Res Center.ac. Agric., Saba basha, Alex. Univ.

Email

-

City

-

Orcid

-

First Name

Faiza

Last Name

M.A El Tawel

MiddleName

-

Affiliation

Plant Physiology Department. Sugar crops Res. Inst., Agric. Res. Center

Email

-

City

-

Orcid

-

First Name

Yusreya

Last Name

H. Tawfik

MiddleName

-

Affiliation

Plant Breeding Department , Sugar crops Res. Inst., Agric. Res. Center

Email

-

City

-

Orcid

-

Volume

28

Article Issue

JULY- SEPTEMBER

Related Issue

394

Issue Date

2007-07-01

Receive Date

2007-08-29

Publish Date

2007-07-01

Page Start

116

Page End

121

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 28_Issue JULY- SEPTEMBER_Pages 116-121.pdf

PDF . 632.3kB

Link

https://asejaiqjsae.journals.ekb.eg/article_1878.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=1878

Order

4

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

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Details

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Article

Created At

22 Jan 2023