The toxicity effects of treating the four chemical and organic fertilizers ‘Animal Manure, Animal Manure + Neem Manure, Potassen and Novatreen' compared with the recommended compound ‘Ortus' were tested on the two-spotted spider mite, Tetranychus urticae Koch and its predatory mite, Phytoseiulus persimilis A.-H. under laboratory conditions. The accumulation mortality percentages of T. urticae and P. persimilis were recorded after 24, 48 and 72 hours from treatment, increased gradually with increasing time of the five aforementioned tested. Comparing between the effects of the five tested compounds on the immatures, adult females and eggs of T. urticae and its toxicity on the predator P. persimilis after24, 48 and 72 hours from treatment were compared. Ortus recorded the highest effect, followed by Potassen and Animal Manure + Neem Manure; while Animal Manure and Novatreen gave the least effect. LT50, LT90 and slop values of T. urticae and its predatory P. Persimilis were calculated, and can be concluded that P. Persimilis gave more tolerance against the five tested compounds than T. urticae.