chickpea (Cicer arietinum L.) is considered a high nutritional value legume crop, due to its high content of total carbohydrates, crude protein, nutrients, and vitamins. Therefore, it is interesting and the requirement to use new microbial genera for biological fertilization for improving the nutritional value, and productivity of chickpea crops. The dual biofertilizers Mesorhizobium ciceri with irradiated Streptomyces alfalfae strain XY25, and with non-irradiated S. alfalfae strain XY25 with the addition of activation nitrogen fertilizer were investigated compared to full dose of chemical fertilization. Moreover, the relation between irradiated S. alfalfae strain XY25 and non-irradiated S. alfalfae strain XY25 with the addition of full NPK chemical fertilizers, in the winter of 2019/2020 was studied. The experiment design was a complete randomized block (RCPD) in triplicate. The results showed that the dual biofertilizers Mesorhizobium ciceri with irradiated and non-irradiated S. alfalfae strain XY25 had positive effect on microbial soil enzymes, dehydrogenase, phosphatase and nitrogenase. Also, they increased the plant's content of phytohormone, micro-elements (Zn, Fe and Mn), phosphorus, potassium, total carbohydrates, and crude protein. All previous improvements due to dual biofertilizers were positively reflected on seed production and the dry weight of 100 seeds.