A FIELD experiment was carried out to investigate the effect of different levels of phosphorus fertilizer application P0 (without P fertilizer), P1 (15 kg P2O5 /fed), P2 (22.5 kg P2O5/fed) and the recommended dose P3 (30 kg P2O5 /fed), either alone or in combination with phosphorus biofertilizer (phosphorus solubilizing bacteria Bacillus megatherium) and foliar spray of micronutrients (Fe + Mn + Zn at mixture rate 1:1:1) on yield, yield components and seed quality of soybean. The results showed that the yield and its components were significantly increased with the levels of mineral P-fertilizers, biological P-fertilizers and foliar spray of micronutrients alone or in combination. Also, the results disclosed that the highest values of yield components obtained with a combination of P rate of 22.5 kg P2O5/fed with inoculation of P-biofertilizer and foliar spray of micronutrients. Application of phosphorus at the rate of 22.5 kg P2O5/fed in combination with phosphorus dissolving bacteria significantly increased the uptake of macro-, micro-nutrients, protein and oil contents. Moreover, the combined application of mineral phosphorus and foliar micronutrients application had a positive effect on N, K, Mn, as well as protein and oil content. The interaction between phosphorus level at 22.5 kg P2O5/fed, phosphorus biofertilizer and foliar micronutrients had a significant influence on uptake of K, Fe, and Mn in seeds of soybean plants. Therefore, it could be concluded that biofertilizers could replace a portion of the chemical fertilizers in soybean production reducing environment problems linked to the mineral fertilizers.