A FIELD experiment was carried out at Arab Elawamr Research Station Farm, Oil Crops Department, Assiut Governorate, Agriculture Research Center (ARC), Egypt, during the summer of 2019 and 2020 season. In order to assess the response of two groundnut varieties (Giza 6 and Sohag 110) to foliar application of some micronutrient (Fe, Zn and Mn) levels cultivated in sandy calcareous soil. The experiment was laid out in randomized complete block design (RCBD) using split-plot arrangement with three replicates. Two groundnut varieties (Giza 6 and Sohag 110) were allocated randomly at the main plot. Four micronutrient (Fe, Zn and Mn) levels (control, 100ppm, 300ppm, 500ppm) were allocated at sub plot. The results showed that there was a significant difference between two the groundnut varieties Sohag 110 suppress Giza 6 at most studied traits in both seasons. The foliar application of micronutrient levels had a significant influence on all studied traits; 300ppm level was superior to all other treatments in both seasons. Also the interaction between varieties and micronutrient levels had a significant impact on growth, yield attributes and yield. The highest mean value of pod yield (4025.2 and 4162.9kg ha-1) and oil yield (1841.9 and 1940.2kg ha-1) were obtained from the interaction between V2 × M2 (300ppm micronutrient on Sohag 110 variety). The micronutrient concentration 500ppm gave the highest value of Fe, Zn and Mn contents in both seed and straw. Saturated and unsaturated fatty acids recorded the maximum value by applied 300ppm micronutrient concentration.