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165498

Direct Shoot Bud Proliferation Protocol from <i>Stevia rebaudiana</i> Leaf Culture for Healthy Biomass Production

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Agronomy

Abstract

AN EFFICIENT and simple regeneration protocol using leaf explants was described on MS medium in the presence of various plant growth regulators. After four weeks of culture, the highest induction of adventitious shoot buds from leaf culture (90%) was achieved on MS medium with 1.0mg/L BA. The largest number of shoot buds (3.02) per leaf explant with no callus formation was achieved on MS medium with 2.0mg/L BA. The best combination for the induction of numerous shoot buds was found to be TDZ + BA + IBA (0.5, 1.0, and 0.5mg/L, respectively), which presented 10.4 shoots per explant with 3.15cm in length. For in vitro root formation stage, Healthy shoots were collected and cultivated on a half-strength MS with various concentrations of NAA and IAA alone or in combination with 2,4-D or IBA. Furthermore, the highest roots (8.17) per shoot with root formation (100 %) was observed using a half-strength MS medium in the presence of 2.0mg/L IAA. Therefore, healthy in vitro rooted plantlets were acclimatized successfully after four weeks in the greenhouse.

DOI

10.21608/agro.2021.66749.1252

Keywords

<i>In vitro</i> rooting, Leaf explant, Plant growth regulators, Plant regeneration, <i>Stevia rebaudiana</i>

Authors

First Name

Ahmed Saad

Last Name

Attaya

MiddleName

-

Affiliation

Department of Plant Production, Faculty of Environmental Agricultural Sciences, Arish University, North Sinai, Egypt

Email

ahmed.attaya@aru.edu.eg

City

-

Orcid

0000-0001-8466-0847

Volume

43

Article Issue

1

Related Issue

25448

Issue Date

2021-05-01

Receive Date

2021-03-18

Publish Date

2021-05-01

Page Start

149

Page End

156

Print ISSN

0379-3575

Online ISSN

2357-0288

Link

https://agro.journals.ekb.eg/article_165498.html

Detail API

https://agro.journals.ekb.eg/service?article_code=165498

Order

12

Type

Short Note

Type Code

1,915

Publication Type

Journal

Publication Title

Egyptian Journal of Agronomy

Publication Link

https://agro.journals.ekb.eg/

MainTitle

Direct Shoot Bud Proliferation Protocol from <i>Stevia rebaudiana</i> Leaf Culture for Healthy Biomass Production

Details

Type

Article

Created At

22 Jan 2023