SUGAR beet (Beta vulgaris L.) is the second source of sugar around the world after sugar cane. The experiment was carried out in the Research Farm , Faculty of Agriculture, Sohag University, in two successive seasons of 2016/2017 and 2017/2018 to study the effect of Nano-micronutrients fertilizer on yield and quality of sugar beet varieties under normal and late sowing conditions. The results showed the early sowing at 21^st October increased the root, quality, sugar and root yields comparing with the late sowing at 21^st November in both seasons. The foliar application of Nano fertilizer treatments affected significantly all studied traits, the foliar application at 60 days after sowing exhibited the higher values of all studied traits comparing with both of foliar application at 105 days and non-fertilizer in both seasons. The four sugar beet varieties (Nabila, Karta, Kosmas and Tesla) differed significantly on all studied traits in the two seasons. Tesla variety was superior than the others of all studied traits. All interaction effects showed significant differences for root fresh weight, sucrose, TSS, purity, root and sugar yields. The highest sucrose % (18.32 and 18.42%) and sugar yield (5.20 and 5.83ton/fed) were obtained from Tesla variety with Nano-fertilized at 60 days from sowing of early sowing in 21st October in 2016/2017 and 2017/2018 seasons respectively. According to principal component analysis, the most appropriate sugar beet varieties for selecting of sugar yield was Tesla variety under the most treatments of foliar Nano- micronutrients in both environments (stable genotype and recommended for the test environments), especially under D1 (sowing at 21 October ) and F2 (spraying time of 60 day after planting).