TWO FIELD experiments were conducted during 2016/2017 and 2017/2018 winter seasons at Fuka Research Station, Faculty of Desert and Environmental Agriculture, Matrouh University, Matrouh Governorate (North West Coast of Egypt, N= 31^o 04 ', E= 27^o 54 '). This work aimed to evaluate the response of three wheat (Triticum aestivum, L.) i.e.; Misr 2, Giza 168 and Sids 12 cultivars under three sowing dates (15th November, 30th November and 15^th December and three treatments of biofertilizer (Control, Blue green algae (BGA) “Gorn 19" and Micobine). A split-split plot design with three replicates was used in both seasons. The main plots were devoted to three wheat cultivars and the sub plots were occupied by the three sowing dates whereas, the sub-sub plots were assigned to the three biofertilizer treatment. Field experiment results indicated that under Matrouh condition, Sids 12 cultivar, gave higher values for plant height, No. of grains/spike, 100 grain weight, biological yield, grain yield and harvest index compare to other cultivars (Misr 2 and Giza 168). In addition, early sowing at Nov, 15 and using BGA as biofertilizer significantly increased all studied characters. Regression analysis for grain yield and yield components, results indicating that the highest effect for delayed sowing was detected for no. of spikes/m² (-1.37 and -1.40) while, the least effect was estimated for 100 grain weight (-0.86 and -1.54) for two seasons, respectively.