FIELD experiments were conducted at the Experimental Farm of Environmental Agricultural Sciences Faculty, Arish University, El-Arish, North Sinai Governorate, during the two growing seasons of 2014/2015 and 2015/2016, the experimental design was split-plot in (RCBD) with three replications. The main plots were devoted to forms of nitrogen and biofertilizer treatments in sub-plots. The main objective to evaluate four nitrogen forms, biofertilizer and their interactions on some growth criteria of sugar beet (Beta vulgaris L.). Results revealed that, urea treatment achieved the highest (LAI) and (RGR) in the first season (CGR) in the second season, whereas ammonium nitrate achieved the highest (CGR) in the first season and (LAI) and (LAD) in the second season. Urea treatment inclusion in seeds with ntrobin application resulted the highest values of (LAI), (CGR) and (LAD) and in the first season. Ammonium sulphate treatment with (phosphorin + ntrobin) obtained the highest (NAR) in the first season. Ammonium sulphate treatment with phosphorin obtained the highest (RGR) in the first season. Ammonium nitrate treatment with phosphorin obtained the highest (LAD) in the second season. Finally, results concluded fertilizing sugar beet plants with ammonium sulphate 100 kg N/fad and inoculated with biofertilizer (ntrobin 600 gm/fad) increased the growth rate sugar beet plants under sandy soil conditions.