THIS INVESTIGATION was carried to assess whether gibberellic acid could be improved suffering beet plants from water deficit under drought. two cultivars of Beta vulgaris L. (Farida and Sultan) and three concentrations of gibberellic acid (50-100-150 mg/l) were used to study their responses to three water regimes (100-75-50 % fc). The experiment was used to determine the tolerance indices, genetic parameters and anatomical and thermal images of various treatments for beet cultivars. The results showed an increase in mean values in studied traits like fresh and dry weight of root, sucrose, proline content, length of stoma and length of stoma pore related with increasing of gibberellic acid concentration up to 150mg/l. Tolerance indices like MP-TOL-YSI-GMP indicated that Farida cultivar was more drought tolerant than Sultan cultivar; and gave the highest yield under the three water regimes at 150 mg/l GA3. Moreover values of genetic parameters; PVC , GVC,GA and h2 showed increasing in number of studied traits of shoot and root can used it for selection in successive breeding program. Recent developments in imaging technology such as thermal imaging, normalized difference vegetative index (NDVI), chlorophyll picture (SPAD reading) and water stress indices were used to agreement particular opportunities to develop robust high-throughput phenotyping. The surface temperature of crop canopies decreases with increasing transpiration as a result of evaporative cooling, therefore, sugar beet cultivars which had high leaves thermal temperature might be drought tolerance. The results, also, indicated that temporal ground-based NDVI was most current for studying the quantitative drought by a significant effect.