TWO FIELD experiments were carried out at the El-Kharga Experimental …. Farm of Desert Research Center Al- Wadi Al- Gadeed Governorate in a sandy clay loam soil, during 2011and 2012, with the aim effect of irrigation water levels and two anti-transpirants agents, (Glycerol and Magnesium carbonate) at two different concentrations (2% and 4%) compared with the control on yield and yields components of sesame (Sesamum indicum L.) cv., Giza 32. Four irrigation water levels were used 110, 90, 70 and 50% from crop evapotranspiration (ETcr) and anti-transpirants. Obtained results was showed that increasing irrigation water levels from 50 up to 110% (ETcr) caused significant increase in the investigated characters such as plant height (cm); 100- seed weight (g); biological yield (kg/fad); seed yield (kg/fad) and oil yield (kg/fad), except protein percentage (%) a clear decreasing. Oil ratio was decreased, as the severity of water deficient increased. In contrast, the protein percentage of seeds increased by increasing water limitation. Glycerol at 4% was increased significantly all parameters plant height (cm), 100-seed weight (g), biological yield (kg/fad), seed yield (kg/fad), oil and protein yields (kg/fad) and better than the other treatments. The highest values for parameters were observed under 110% ETcr irrigation water levels and Glycerol, at 4%. On the other hands, seed yield (kg/fad) reduction under severe deficient (50% ETcr) conditions and the control anti-transpirants.