THE OBJECTIVES of this study were to assess thirty-seven white Lupin (Lupinus albus L.) accessions that collected from different regions in Egypt based on fifteen morphological traits and cytological characterization. Biplot shows that time of green ripening, time of ripening, pod length and growth habit are valuable to distinguish lupine genotypes. The studied lupine genotypes categorized into two groups. The first group contains most of genotypes collected from Upper Egypt, while the second group contains the genotypes of Lower Egypt. The genotype collected from Middle Egypt (Giza, El-Fayoum, Beni-Souif and El Menia) are scattered in the two large group. The genotype collected from North of Giza are more related to the Lower Egypt group, while, the other genotypes collected from Middle Egypt (South Giza, El-Fayoum, Beni-Souif and El Menia) are more related to Upper Egypt group. The accession number 18 recorded the high value (10.11) of chromosomal aberration, especially, laggards, fragments and stickiness should be neglected from further selection for breeding program.