This work had done to assess the toxic and impact of  Closer    24 % SC  (sulfoxaflor) and Uphold 36% SC (Spinetoram 6% + Methoxyfenozide 30%),  considered as effective and selective insecticides for controlling especially of E. insulana under laboratory conditions where the two insecticides are a commercially produced considered being a valuable insecticides control agent of many  insects.  The   compatibility of these two control strategies was tested by evaluating the lethal effects resulting from ingestion of Uphold and Closer residues on treated E. insulana under laboratory conditions. The results revealed that LC_50s were 0.349 and 1.090 ppm, when larvae treated with closer and uphold, respectively. The results exhibited a significant prolonged in duration two stages larval (25.3 and 20.6 days/larvae) and pupal formed (11.6 and 14.6 days/pupa) resulted from two tested compounds, respectively, the two tested compound caused significantly prolonged preoviposition and shortened oviposition periods and decreased pupation, adult emergence and hatchability percentages. Treatment with uphold and closer caused significally decreased in total lipid 6.93&4.31 (mg/g.b. wt) compared with un treated 8.97(mg/g.b.wt) and also reduction in chitinase and N- acytel-glucoseamine of the two tested compounds (46.97&58.19 µg NAGA x103/min/g.b.wt) and (97.60 &112.90µg NAGA /g.b.wt) compared with control (87.80&173.90), respectively. The deformation histological observations in the mid gut and fat body of E. insulana resulted that this instar is very sensitive to the closer tested compound. Modifications in the mid-gut and visible feature changes in volume, morphology and deformation in the midgut epithelium. The epithelial of the midgut appears flimsy and is dominated by asymmetrical and abnormal morphologically formations of goblet cells compared to the untreated larvae.