In our study, laboratory trials to determine the effectiveness of Mentha pulegium leaves essential oil nanoparticles against the pink and spiny Bollworms, Pectinophora gossypiella (Saunders) and Earias insulana. M. pulegium was analyzed by gas mass spectroscopy (GC/ MS) for the identification of the chemical composition of the essential oil. Three major active components were identified as D-Carvone (28.46%), Menthol (27.88%) and l-Menthone (15.92%) the oil constituent. The level of LC values, P. gossypiella exhibited more sensitivity to M. pulegium than E. insulana and the mortality rate increased by increasing the exposure time. A significant percent of larval mortality and malformation were recorded (81.113 and 68.89%) in addition to a remarkable percent of pupal mortality and malformation reached 30.55 and 34.53%, with a significant reduction in adult emergence for P. gossypiella and E. insulana, respectively. Moreover, some histological deformations at the level of the cuticular layer of treated larvae of both insects as a result of M. pulegium treatment were also detected compared to the control check.