The main efforts of scientists to reduce the appliance of nematicides that are commonly found in the markets led to an increase in the demand for safe, effective and environment-friendly alternatives. Hence, the present research aims to test three safe and effective substances, Humic acid (HA) concentration of 1500 ppm; Mychorrhizal fungi, Glomus mosseae and the commercial nematicides Bio-zeid (Trichoderma album) half dose compared with the nematicide Vydate on root-knot nematodes Meloidogyne javanica infected tomato plants, as well as its effects on the vegetative characteristics of tomato plants. The obtained results revealed that all tested materials decreased M. javanica J₂ population density. The triple treatment (Humic acid + G. mosseae + Bio-zeid (half dose)) gave the best results, as it led to a decrease in the number of M. javanica J₂ by 88.0%  more than that occurred by the chemical nematicide Vydate, which gave a reduction percentage of 87.1%. The triple treatment also led to a decrease in the numbers of root gall index by 80.0%. As well as triple treatment also improved the vegetative characteristics of tomato plants, it caused an increase in plant height, shoot weight and root weight by percentages (53.0, 29.0 and 148.1%) while contrariwise occurred by an appliance with Vydate (42.9, 21.4 and 92.6%), respectively, compared to the control. Thus, triple treatments can be recommended as a cheaper and safer alternative for plants and humans instead of chemical nematicides in integrated nematode control programs