Photosensitizers are promising chemical compounds in agriculture sectors. In this investigation, our discussion is focused on three different photosensitizer and their effects on the main metabolites, total carbohydrates, total proteins, and total lipid were determined in the total body homogenates of Spodoptera littoralis. The results showed the highest decrease in the total lipid. On the other hand, total carbohydrates and protein showed a lower reduction in most treatments. The Rose Bengal photosensitizer was more effective on death percentages of Spodoptera littoralis and lightly played an active role in enzyme activity