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-Abstract
Toxicological, histopathological and ultrastructure studies of Metarhizium anisopliae and Purpureocillium lilacinum infected Aphis craccivora nymph were investigated in this study. comparison between fungal spore suspensions and fungal culture filterates virulence effects of the two tested entomopathogenic fungi against A. craccivora nymph were done under laboratory condition and the toxicological bioassay results revealed that P.lilacinum has a high toxic effect than M. anisopliae in which the LC50 of fungal spore suspensions and culture filterates after 7 days post-treatment was (7×102, 1×105 spore/ml and 3.2, 4.7 % ) for P. lilacinum and M.anisopliae, respectively. Treated nymph with spore suspensions was examined using both light and transmission electron microscope to study histopathological and ultrastructure changes compared with the control one. This comparison suggested that there is a deformation of the nymph body, complete destruction of all internal organs with an extensive invasion of the fungal spores throughout all internal body regions.
DOI
10.21608/eajbsf.2020.97626
Keywords
Black aphid bean, Aphis craccivora, Biocontrol, Metarhizium anisopliae, Purpureocillium lilacinum, Histopathological, ultrastructure studies
Authors
Affiliation
Botany and Microbiology department, Faculty of Science, Suez University
Email
shemoo_1000@yahoo.com
Orcid
-Affiliation
Plant Protection Research Institute Agriculture Research Center Dokki Giza Egypt*
Email
dryasmeinsayed@gmail.com
City
-Orcid
-Affiliation
Plant Protection Research Institute Agriculture Research Center Dokki Giza Egypt
Email
drsuzanelrefaey@yahoo.com
City
-Orcid
-Link
https://eajbsf.journals.ekb.eg/article_97626.html
Detail API
https://eajbsf.journals.ekb.eg/service?article_code=97626
Publication Title
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
Publication Link
https://eajbsf.journals.ekb.eg/
MainTitle
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