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Pathogenic and Lethal Effects of Some Entomopathogenic Nematodes Species against the Greater Wax Moth, Galleria mellonella, (L.) Larvae (Lepidoptera: Galleridae )

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Last updated: 01 Jan 2025

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Abstract

Laboratory experiments were performed to evaluate the pathogenic and lethal effects of the genera entomopathogenic nematodes, (EPNs) (Heterorhabditis bacteriophora Poinar HP88) and Sc (Steinernema carpocapsae All strains, (Steinernema carpocapsae, Steinernema. scapterisi and Steinernema. glaseri) on greater wax moth larvae, Galleria mellonella, at the three concentrations; 20IJs, 50IJs and100IJs (infective juveniles) after different times, 6, 12, 24 and 48hrs of exposure times. The mortality percentage of EPNs was determined on G.mellonella larvaefor each concentration. It was found that the infectivity of nematode strains against G. mellonella larvae was concentration-dependent; i.e. the mortality percentage increased as the infective juvenile concentrations increased. The mortality percentages among G. mellonella larvae after 48hrs post-treatment by all EPNs strains were highly significant increased at 100 IJs/L for each concentration.  The treatments at 20 IJ/L noticed that, LT25 was 10.17hrs for G. mellonella larvae treated with S. carpocapsae after48hrs post-treatment.Also, results indicated S. glaseri gave the highest mortality rate for the tested larvae reached to 96.66 %, followed by S. carpocapsae achieved 96.16%, but the H. bacteriophora HP88 recorded 84.24 % mortality after four times of exposure.  Meanwhile at 50IJ/L data cleared that, LT25 value was 7.36hrs for G. mellonella larvae treated with S. carpocapsae while S. carpocapsae  treatment gave the highest significant mortality for the tested larvae reached to 94.45 % followed by 94.25% for S. glaseri, but H. bacteriophora recorded 86.01 % mortality. The results of the study noticed that, LT25 value was 3.26hrs for G. mellonella larvae treated with S. scapterisi also showed that S. carpocapsae treatment gave the highest significant mortality for the tested wax moth larvae recorded 98.18 % followed by S. glaseri achieved 98.09% and the H. bacteriophora recorded 92.77 % mortality at 100IJ/L after 48hrs of treatments. Also, data showed a strong coefficient correlation between the testedENPs against G. mellonella larvae.  Also, there were inverse relationship between the times of infection   and concentrations. Our results suggest that H. bacteriophora, S. carpocapsae, S. scapterisi and S. glaseri can be used as valuable tools in biological control programs oflast instar larvae of G. mellonella.

DOI

10.21608/eajbsf.2019.43591

Keywords

Galleria mellonella, Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema scapterisci, Steinernema glaseri, mortality, toxicity

Authors

First Name

Shoukry

Last Name

F.

MiddleName

I.

Affiliation

Faculty of Science, Zagazig University

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City

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Orcid

-

First Name

Ahmed

Last Name

A.

MiddleName

F.

Affiliation

Faculty of Science, Zagazig University

Email

-

City

-

Orcid

-

First Name

Khater

Last Name

S.

MiddleName

K.

Affiliation

Faculty of Science, Zagazig University

Email

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City

-

Orcid

-

First Name

El-Lakwah

Last Name

F.

MiddleName

S.

Affiliation

Plant Protection Research Institute, Dokki, Giza, Egypt

Email

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City

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Orcid

-

First Name

Abd-Elmonem

Last Name

M.

MiddleName

H.

Affiliation

Plant Protection Research Institute, Dokki, Giza, Egypt

Email

hendabdelmonem1111@gmail.com

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-

Orcid

-

Volume

11

Article Issue

2

Related Issue

5683

Issue Date

2019-06-01

Receive Date

2019-06-01

Publish Date

2019-06-01

Page Start

69

Page End

83

Print ISSN

2090-0864

Online ISSN

2090-0791

Article File

EAJBSF_Volume 11_Issue 2_Pages 69-83.pdf

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https://eajbsf.journals.ekb.eg/article_43591.html

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https://eajbsf.journals.ekb.eg/service?article_code=43591

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7

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Original Article

Type Code

6

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Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control

Publication Link

https://eajbsf.journals.ekb.eg/

MainTitle

Pathogenic and Lethal Effects of Some Entomopathogenic Nematodes Species against the Greater Wax Moth, Galleria mellonella, (L.) Larvae (Lepidoptera: Galleridae )

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Article

Created At

22 Jan 2023